This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter "CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Global climate change affects crop production through altered weather patterns and increased environmental stresses. Such stresses include soil salinity, drought, flooding, metal/metalloid toxicity, pollution, and extreme temperatures. The variability of these environmental conditions pared with the sessile lifestyle of plants contribute to high exposure to these stress factors. Increasing tolerance of crop plants to abiotic stresses is needed to fulfill increased food needs of the population. This book focuses on methods of improving plants tolerance to abiotic stresses. It provides information on how protective agents, including exogenous phytoprotectants, can mitigate abiotic stressors affecting plants. The application of various phytoprotectants has become one of the most effective approaches in enhancing the tolerance of plants to these stresses. Phytoprotectants are discussed in detail including information on osmoprotectants, antioxidants, phytohormones, nitric oxide, polyamines, amino acids, and nutrient elements of plants. Providing a valuable resource of information on phytoprotectants, this book is useful in diverse areas of life sciences including agronomy, plant physiology, cell biology, environmental sciences, and biotechnology.

Global climate change affects crop production through altered weather patterns and increased environmental stresses. Such stresses include soil salinity, drought, flooding, metal/metalloid toxicity, pollution, and extreme temperatures. The variability of these environmental conditions pared with the sessile lifestyle of plants contribute to high exposure to these stress factors. Increasing tolerance of crop plants to abiotic stresses is needed to fulfill increased food needs of the population. This book focuses on methods of improving plants tolerance to abiotic stresses. It provides information on how protective agents, including exogenous phytoprotectants, can mitigate abiotic stressors affecting plants. The application of various phytoprotectants has become one of the most effective approaches in enhancing the tolerance of plants to these stresses. Phytoprotectants are discussed in detail including information on osmoprotectants, antioxidants, phytohormones, nitric oxide, polyamines, amino acids, and nutrient elements of plants. Providing a valuable resource of information on phytoprotectants, this book is useful in diverse areas of life sciences including agronomy, plant physiology, cell biology, environmental sciences, and biotechnology.

The third volume of the series ‘Bacilli and Agrobiotechnology’ is comprised of 25 chapters that bring a unique perspective to the readers about Bacillus-mediated biotic and abiotic plant stress tolerance, bioremediation and bioprospecting. These chapters are prepared by the leading scientists of global repute. The negative impacts of agrochemicals such as chemical fertilizers and pesticides on human health and environment are paramount. Bacillus and allied genera of beneficial plant-associated microbes are presenting beacon of hope to the farmers, plant scientists and stewards of environment. Several chapters of this volume focus on the induction of various signaling pathways in plants by Bacillus spp. to alleviate biotic and abiotic stresses impacted by global climate change Agricultural lands contaminated with heavy metals affect the ecological food chain starting from crop cultivation. How the toxic effects of trace metals originating from industrial effluents and agrochemicals can be remediated? This book addresses how to overcome these issues by applying elite strains of Bacillus. Bioprospecting is a systematic and organized search for conversion of bioresources to industrially important products by utilizing microbe-derived metabolites. This volume is enriched by including the bioprospecting aspects mediated by Bacillus spp. with novel insights.

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter "CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Feeding of powdered black seeds of historically famous medicinal herb, Nigella sativa L. has been shown to lower serum triglycerides and decrease egg cholesterol in laying hens. This study aimed to investigate effects of varying doses of N. sativa seed powder (0, 1.5, 2.5 and 3.0%) and acetone extracts (0, 1.5 and 3.0%) on feed intake, health, fat content, serum lipid profiles and population of intestinal microflora of broiler. Results revealed that N. sativa supplemented feed had no significant effects on feed intake, body weight, fat content and mortality rate. However, supplementation of 3.0% seed powder and 0.4% extract of N. sativa significantly (p

Phosphate predominantly present as inorganic compounds, which are either calcium or iron and aluminum salts. Iron and aluminum phosphates predominate under acidic condition. The low availability of P to plants is because the vast majority of soil P is found in insoluble forms .Thus, the release of insoluble and fixed forms of phosphorus is an important aspect of increasing soil phosphorus availability.Plant root-associated phosphate solubilizing bacteria (PSB) have been considered as one of the possible alternatives for inorganic phosphate fertilizers for promoting plant growth and yield. Considering the above stated role of bacteria for P solubilization in acid soils of Bangladesh, the present work was therefore undertaken with the following objectives: To isolate some rhizoplane bacteria from the roots of rice plants and screening potential phosphate solubilizing bacteria (PBS) from the isolates; To quantify P solubilization from tricalcium phosphate by potential PSB in broth culture conditions; and To see the performances of potential PSB on growth and nutrients content of rice in acidic soil of Bangladesh.